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PRPmedKnowledge & Tools

Scientific tool for healthcare professionals

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Tool for healthcare professionals

PRP Classification & Reporting

This tool classifies entered PRP parameters according to published classification systems. Complete classification codes are shown only when the information required by the respective system is available. Missing or ambiguous components are visibly marked as partial classifications and are not estimated.

Units
1 G/L = 1,000 /µL · 1 T/L = 1 million/µL
01

Measurements

Cell counts before and after centrifugation
Whole blood · baselinebefore centrifugation
PRP preparationafter centrifugation
02

Preparation and application

yellow outline = not yet answered
Exogenous activationPAW · Mishra · PLRA · DEPA · MARSPILL
Activation methodrequired by PLRA when “yes”
Centrifugation stepsMARSPILL
Preparation methodMARSPILL · handmade or automated
Image-guided applicationMARSPILL
PhotoactivationMARSPILL
PRP formatForm
Declaration “minimal/no WBC”Mishra · only if stated by study or manufacturer
Centrifugation protocoloptional · for methods reporting
Information for Ehrenfest 2009not calculable

Ehrenfest distinguishes preparations by leukocyte content and fibrin architecture but gives no numerical threshold for “leukocytes present”. This therefore cannot be derived from cell counts and must be specified by the user. Without both entries, the Ehrenfest classification remains unavailable.

Does the preparation contain leukocytes?Ehrenfest
Fibrin architectureEhrenfest · PRP or PRF
03

Derived metrics

Concentration factor
×

Platelets relative to baseline

DEPA dose
bn

DEPA: calculated from total volume obtained

Applied dose
bn

calculated from the actual applied volume

Recovery
%

platelet yield from whole blood

Purity
%

platelet fraction of all cells

Cellular compositionFractions of all cellular components
Whole blood
PRP
Platelets Leukocytes Red blood cells
04

Classification map

Position relative to thresholds
> BaselinePAW A · MARSPILL Lc-R
= BaselinePAW B · MARSPILL undefined
< BaselinePAW B · MARSPILL Lc-P

05

Classification by six systems

Status and rationale for each system
06

Methods information for manuscript preparation

complete classifications only
07

Primary sources

  1. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27(3):158–167. doi:10.1016/j.tibtech.2008.11.009
  2. Mishra A, Harmon K, Woodall J, Vieira A. Sports medicine applications of platelet rich plasma. Curr Pharm Biotechnol 2012;13(7):1185–1195. doi:10.2174/138920112800624283
  3. DeLong JM, Russell RP, Mazzocca AD. Platelet-rich plasma: the PAW classification system. Arthroscopy 2012;28(7):998–1009. doi:10.1016/j.arthro.2012.04.148
  4. Mautner K, Malanga GA, Smith J, et al. A call for a standard classification system for future biologic research: the rationale for new PRP nomenclature. PM&R 2015;7(4 Suppl):S53–S59. doi:10.1016/j.pmrj.2015.02.005
  5. Magalon J, Chateau AL, Bertrand B, et al. DEPA classification: a proposal for standardising PRP use and a retrospective application of available devices. BMJ Open Sport Exerc Med 2016;2(1):e000060. doi:10.1136/bmjsem-2015-000060
  6. Lana JFSD, Purita J, Paulus C, et al. Contributions for classification of platelet rich plasma – proposal of a new classification: MARSPILL. Regen Med 2017;12(5):565–574. doi:10.2217/rme-2017-0042

Where the original publications are ambiguous. This tool does not close such gaps by making its own definitions; it makes them visible. Three examples: PLRA gives no denominator for the 1% leukocyte and red blood cell thresholds, so L and R remain open. DEPA names one component dose of injected platelets but calculates it from the total volume obtained in the original paper; both values are therefore shown separately. MARSPILL defines Lc-R as above and Lc-P as below baseline, so exact equality falls into neither category, whereas PAW assigns the same case unambiguously to B.

Intended purpose. This tool is intended exclusively for the scientific characterisation and documentation of entered measurements. It makes no statement about efficacy, suitability or indication and provides no diagnosis or patient-specific treatment, dosing or therapeutic recommendation. It does not replace the manufacturer’s instructions for the system used or professional assessment by qualified healthcare personnel.