PRP Tubes and Centrifuges: Compatibility, Best Settings and PRP Protocol

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PRP Tubes and Centrifuges: Compatibility, Best Settings and PRP Protocol

PRP tubes and centrifuges: compatibility & optimal settings

The collection of platelet-rich plasma (PRP) requires not only the right combination of PRP tubes and centrifuge, but also adherence to a clearly defined PRP protocol. This protocol defines the individual steps of PRP production - from blood collection to centrifugation and application. In this article, you will learn all about the importance of the right centrifuge, the compatibility of PRP tubes with common models, the best centrifugation settings and what an optimal PRP protocol looks like.

1. Importance of the right centrifuge for PRP collection

The centrifuge is an essential component of the PRP protocol. It separates the blood components and ensures the concentration of platelets in the plasma. A precise centrifuge ensures the quality of the PRP obtained and the effectiveness of the therapy.

PRP centrifuge

Why the centrifuge is part of the PRP protocol:

  • Efficient separation: The centrifuge separates the blood into its components (red blood cells, white blood cells and plasma) and isolates the platelet-rich plasma.
  • Consistency: Adherence to a standardized protocol with precise centrifuge settings ensures reproducible results.
  • Platelet protection: Incorrect settings can damage the delicate platelets and affect the quality of the PRP.

A high-quality centrifuge suitable for PRP enables clean and efficient processing and is therefore an essential part of any PRP protocol.

2. Compatibility of PRP tubes with common centrifuge models

As part of a standardized PRP protocol, the compatibility of PRP tubes with the centrifuge used is crucial. Not all tubes fit every centrifuge and choosing the wrong tube can affect the quality of the PRP.

Important factors for compatibility:

Tube size and shape:

  • Standard PRP tubes have a volume of 9 to 10 ml. They must fit perfectly into the holders of the centrifuge to ensure uniform separation.
  • Common models such as the Hettich EBA 200 or the DUO centrifuge are specially designed for tubes of this size.

Material of the tubes:

  • Glass tubes: Chemically stable and biocompatible, but sensitive to high rotational speeds.
  • Plastic tubes: Stronger and lighter, but some contain micronized silicone that could interfere with platelet activation.

Separating gel and anticoagulant:

  • Tubes with release gel (e.g. Vi PRP-PRO PRP tubes) are ideal for a clean separation of the blood components.
  • The anticoagulant (sodium citrate or heparin) in the tubes should be coordinated with the protocol.

Rotor type of the centrifuge:

Angled rotors: Suitable for tubes with separating gel.

Swivel rotors: Ideal for applications where maximum PRP purity is required.

Checklist for compatibility:

  • Do the tubes fit into the rotor of the centrifuge?
  • Can the tubes withstand the planned centrifugal force?
  • Does the tube support the desired PRP protocol (e.g. separating gel or specific anticoagulant)?

3. Optimal centrifugation settings for maximum PRP yield

Centrifugation is a central component of the PRP protocol. The correct settings (rotation speed, centrifugal force and duration) are crucial in order to achieve the highest platelet concentration.

Recommended settings for PRP

Recommended settings for PRP

The following table shows the recommended settings for centrifugation to obtain platelet-rich plasma (PRP):

Parameters Recommended value
Rotation speed (RPM) 3.400 - 4,000 rpm
Centrifugal force (RCF) 1.200 - 1.500 g
Duration of centrifugation 6 - 10 minutes

Explanation of the parameters:

RPM and RCF:

  • The rotational speed(RPM) and the relative centrifugal force(RCF) influence how efficiently the blood components are separated.
  • Example: With the Hettich EBA 200 a speed of 3,400 RPM corresponds to an RCF of approximately 1,200 g.

Duration:

  • Too short a centrifugation will result in incomplete separation, while too long a duration may impair platelet activation.

Tubes with separating gel:

  • Separating gel ensures clean separation of plasma and cellular components. Without separating gel, manual separation is necessary, which requires more experience.

The centrifugal force (RCF) is crucial as it influences the separation of the blood components. To calculate the RCF, use the formula or a converter

Calculator: Relative Centrifugal Force (RCF)

RCF: -

Calculator: Revolutions per minute (RPM)

RPM: -

4. The perfect PRP protocol: step by step

A PRP protocol describes the standardized steps required to obtain and apply PRP. Here is a tried and tested protocol:

Step-by-step instructions:

  • Blood collection:

    • Draw 9-10 ml of venous blood into a PRP tube with sodium citrate as anticoagulant.

  • Centrifugation:

    • Set the centrifuge to 3,400 RPM (or 1,200 g) for a duration of 6-10 minutes.
    • Use tubes with separating gel to ensure clean separation.

  • Collection of the PRP:

    • After centrifugation, the red blood cells are at the bottom, the PRP at the top and the separating gel in between.
    • Withdraw the platelet-rich plasma (PRP) using a sterile syringe.

  • Application:

    • Inject the PRP directly into the desired tissue (e.g. skin, joints) or use it for aesthetic treatments such as microneedling.

  • Optional:

    • For specific applications, PRP can be combined with other substances (e.g. melatonin for hair treatments).

5. Frequently asked questions about the PRP protocol

1. What is the difference between PRP with sodium citrate and heparin?

  • Sodium citrate: Prevents blood clotting and ensures that the growth factors are only released during application.
  • Heparin: Can activate the blood platelets too early, which reduces the effectiveness of the PRP. Sodium citrate is therefore preferred.

In the European Union, only sodium citrate is used as an anticoagulant in PRP tubes with separating gel for both regulatory and safety reasons.

2. Can any centrifuge model be used for PRP?

No, the centrifuge must be compatible with the PRP tubes and have adjustable parameters (RPM, RCF and duration).

3. How do I ensure that the PRP is of high quality?

Adhere to a standardized PRP protocol, use compatible tubes and centrifuges and carefully follow the recommended settings.

Conclusion: PRP tubes, centrifuges and the right PRP protocol

The right combination of PRP tubes and centrifuge as well as the optimal centrifugation settings are crucial for the quality of PRP collection. Compatible tubes, precise centrifuges and adherence to recommended settings ensure maximum platelet concentration and thus the success of PRP therapy.

By paying attention to these factors, you can not only achieve consistent results, but also maximize the safety and efficacy of your treatments. Use the tube and centrifuge manufacturers' recommendations to provide your patients with the best possible therapy.

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