Centrifuge

DUO PRF/PRP centrifuge - Class IIa medical device for PRP and PRF therapies

100401
Certified Class IIa medical device – specifically designed for PRF and PRP therapies. Maximum speed of 4500 RPM and RCF up to 2490 x g for precise and safe blood preparation. Quiet operation at only 56 dB – ideal for use in quiet clinic or practice environments. User-friendly controls with pre-set programs and easy parameter adjustments. Highest...
immediately available
€1,679.83

PRP Power Kit Pro - your complete set for professional PRP treatments

100399
This set includes: 1x DUO Centrifuge2x PRP Tubes | Vi PRP-Pro VPE 10 pcs1x KIPIC® Needle 25G x 42mm, VPE 100 pcs1x KIPIC® Mesotherapy Needle 30G 4mm | VPE 100 pcs1x KIPIC® Mesotherapy Needle 32G x 4mm | VPE 100 pcs1x Mediware Disposable Syringes 5ml 3-part Luer-Lock Sterile (VPE 100 pcs)1x Mediware Disposable Syringes 2/3ml 3-part Luer-Lock Sterile (VPE...
immediately available
€2,047.67

Centrifuge Hettich EBA 200

100347
Big performance for the small lab. The Hettich EBA 200 small centrifuge is a practical laboratory centrifuge for small sample volumes.   *Information on the calculation of the delivery date  
immediately available
€1,088.24

PRP Starter Set | EBA 200

100400
PRP starter set consisting of:   1x Hettich EBA 200 centrifuge  2x PRP tubes | Vi PRP-Pro PU 10 pcs.  1x KIPIC® needle 25G x 42mm, PU 100 pcs. 1x KIPIC® Mesotherapy Needle 30G 4mm | PU 100 pcs. 1x KIPIC® Mesotherapy Needle 32Gx4mm | PU 100 pcs. 1x Mediware disposable syringes 5ml 3-piece Luer-Lock sterile (PU 100 pcs.) 1x Safety Blood Collection...
immediately available
€1,318.49

Centrifuge Hettich EBA 270

100359
Hettich EBA 270 is a small centrifuge with swing-out rotor, which is especially suitable for medical practices. Centrifuge conventional laboratory tubes to 15 ml at a maximum speed of 4,000 rpm. This corresponds to an RCF of 2254. Due to their 90° rotating rotor, they are particularly suitable for centrifuging laboratory tubes with separating gel.  
immediately available
€1,147.06
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Which centrifuge for PRP?

A "practice centrifuge" is required for the use of PRP in medicine. "Practice centrifuges" are generally referred to as centrifuges that are used for therapeutic treatments, for example for the production of autologous blood preparations. These centrifuges are therefore classified as class 2a medical devices.

The production of platelet-rich plasma (PRP) from whole blood requires centrifugation to isolate and concentrate platelets. Centrifugation uses the principle of centrifugal force to accelerate the natural sedimentation process. Practitioners who administer PRP with a significant concentration of viable platelets into damaged tissue see increased tissue regeneration and better results.

PRP preparation

Blood consists of red blood cells, white blood cells, blood plasma and platelets. If you were to place a vial of blood on a table, the blood would eventually separate into three different layers, with the heaviest parts sinking to the bottom and the lightest rising to the top. Eventually, three distinct layers would become visible: red blood cells (RBCs) at the base, white blood cells and platelets in a very thin layer above the RBCs (also called the "buffy coat"), and finally platelet-poor plasma at the top.

Centrifugation accelerates this innate deposition process and allows physicians to isolate and re-inject platelets very quickly. Under centrifugation, the particles in suspension experience a radial force that moves them away from the axis of rotation. Particles with the closest density move faster and are separated from less dense and smaller particles.

Platelets are cells in our body that contain nutrients and growth factors that stimulate normal wound healing, just as the skin heals after a scratch. A small amount of blood is drawn from the patient using PRP tubes to produce PRP. Once the blood is drawn, the tubes are placed in a PRP centrifuge, which spins the blood at high speed to separate the blood into concentrated red blood cells and platelets; once the blood is separated, the red blood cells are discarded and we are left with concentrated PRP (platelet-rich plasma), which is used in the treatment process.

Best PRP centrifuge rates and protocols

Calculating the exact speed, force and time required to stratify the blood requires a thorough understanding of physics. Many factors influence the centrifugation process, including the width, length and weight of the PRP kit, as well as the speed and force exerted by the centrifuge. In addition, the centrifugal force increases with the distance from the axis of rotation. Therefore, a larger centrifuge exerts more force at the same speed than a smaller centrifuge.

The relative centrifugal force is an expression of the gforce, the gravitational force of the earth. When calculating the gforce, it should be noted that the gforce acting on the particles is exponential to the rotational speed. This exponential value is expressed in revolutions per minute (rpm). The exponential value is particularly important when considering the spin speed; for example, doubling the speed quadruples the force.

Commercially available centrifuges are designed to maximize sedimentation and isolate platelets when used with their respective PRP tubes. Due to the varying weight and size of PRP tubes, some kits require a dedicated centrifuge and the manufacturer will establish protocols to optimize the sedimentation process based on the exact size of the PRP tube.

Centrifugation and temperature

The most important factors to consider during centrifugation are time, speed and temperature, but few PRP centrifuges on the market have temperature control.

Does this monitoring of the sector lead to different results in orthobiology? Temperature is an essential variable that must be taken into account when working with biological material. The high speeds generate friction and can heat the sample during centrifugation.

Temperature control becomes even more important when centrifugation is performed outside the vacuum. Centrifugation outside the vacuum leads to additional resistance and temperature rise. Research biologists traditionally cool their centrifuges to compensate for the temperature rise caused by friction.

If the sample is heated during centrifugation, the platelets may be activated prematurely. In addition, the heat could damage the platelets and reduce their vitality.

Cooling the centrifuge slows down the biological processes and can help to maintain the vitality of the platelets. This known biological phenomenon has been recognized by researchers as increased platelet activation and the release of alpha¹ granules, but rarely as damage to platelet viability during centrifugation.

Reproducibility of results when working with biological material depends on precise control of variables, and inconsistent results in orthobiology could be related to different sample temperatures. The best PRP protocols depend on the exact specifications of the PRP kit. The ideal centrifugation speed and time is calculated based on the mass and size of the PRP tube.

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